Automated DNA Extraction: RT-PCR Analysis of GMOs, Allergens, and Pathogens in Food

Event organized by R-Biopharm Italia Srl.

The R-Biopharm International Group has been active in the development and supply of analytical solutions for food and feed diagnostics for over 35 years.
The issue of food allergens is critically important to food safety and requires precise and reliable analytical methods to ensure products are safe for allergic consumers.
R-Biopharm offers various analytical techniques to support allergen management systems, each tailored to meet the specific needs of manufacturers, consumers, and regulatory bodies.
Allergen monitoring begins with the inspection of raw materials, including sourcing, transportation, and storage. It continues inside production facilities, where cleaning and sanitation procedures are applied to production lines and verified through surface and wash water analyses.
Finally, the process involves testing performed by service or official laboratories using accredited analytical methods.
R-Biopharm's portfolio includes various analytical technologies:

• Lateral flow tests with the bioavid and RIDA®QUICK lines: a qualitative, easy-to-use, rapid, and cost-effective method, ideal for on-the-spot allergen detection.
• ELISA immunoassay with the RIDASCREEN® line: a quantitative method that allows direct detection of allergenic proteins. This sensitive and automatable technology offers accurate evaluation of allergen levels in tested samples.
• Molecular biology with the SureFood® line: a qualitative and quantitative method based on DNA extraction and amplification using advanced instruments. It ensures precise allergen detection and quantification in samples.

R-Biopharm’s analytical solutions cover a wide range of needs for allergen management in the food industry. The variety of technologies offered enables accurate and comprehensive allergen monitoring throughout the entire food chain, ensuring food safety for producers, consumers, and quality control professionals.

Session content

Molecular Analysis via Real-Time PCR is an essential tool for quality control and food safety, with applications in various areas including the detection of GMOs, allergens, and pathogenic microorganisms.

The precision and reliability of such analyses heavily depend on the quality of nucleic acid (DNA/RNA) extraction—a critical step that significantly influences the sensitivity, reproducibility, and robustness of the entire analytical method.

In this context, automating the extraction process emerges as an effective strategy to enhance protocol standardization, reduce operating times, and minimize the risk of errors and cross-contamination.

R-Biopharm offers compact and versatile automated DNA/RNA extractors, easily integrable into the routine workflows of molecular biology laboratories. These solutions are designed to provide flexibility and meet high-quality standards, contributing to improved analytical efficiency and optimized workflow management.

During the session, practical applications will be presented in key contexts such as the detection of GMOs and allergens in food (both raw materials and finished products), as well as the monitoring of foodborne and environmental pathogens, including Listeria, Salmonella, STEC, and Legionella.

The event will be held in Italian